Reasons behind liking towards Benchtops ( with gas mixers ) 1. Its recovery time is fast. 2. You can adjust the gas concentrations according to your need ( what you can not do with simple warmer like benchtops). 3.Its a direct heat transfer technology,so regaining the temperature is fast. 4. Upper lid is also warmed and slightly higher than the base ( so evaporation is is less hence change in osmolality of media in long term culture will be less,( hence no need of waterbody, maybe similar to embryoscope,which I don’t have. 5. Multiple individual chambers for each patient ( we can keep them exclusively for one patient). 6. Occupies less space in lab. We get hatching blasts in my Benchtop, we get pregnancies too,but compared to blast transfers of both system, conventional gave better pregnancy rates . Questions is :Can we improve it more or similar to conventional,. We are doing this study from pre-equilibration to day5 with sibling oocytes in both systems, with same gas concentrations ( CO2 concentration is set according to the pH measured in conventional one). I am trying to get ideas from my fellow IVF professionals and embryomailers ( in other forum). I received a beautiful paper on various incubation system ,(but still less with benchtops with gas mixers), which I can share on request.
A documentary made by one of my French patient,Isabell Stroh. She writes in details about us and the city and the culture and ambience.
A patient with severe hirsutism and PCOD. Under gone thrice IVF, first two cancelled because of ,first no stimulation and then hyperstimulation. Thiird attempt: stimulation for 6 days and Hcg when follicles are small. one egg retrieved…In-vitro matured for two days then ICSI ied ..Led to Pregnancy, Developed severe diabetes in early pregnancy ,partially controlled by Insulin,but aborted at 20 weeks.
Now she is again referred from Nagpur,Fourth attempt…no eggs
Fifth attempt : stimulation for 7 days ( FSH and GnRhanta). then hCG…three immature eggs ( see vedio) ,.In-Vitro matured for two days…Reached MII stage..ICSI ied by Dr. Rinku Banerji , ….Embryos transfered on day 3( 24th Feb 2014)immature eggs of Sumedha Chaubey_xvid_001 ( See Video)….Waiting for the result